C60 Oil for Floxing
What is C60? And, how has some floxies used C60 in the past for symptom remediation? We will look at these questions and more in this article which looks at how some have used C60 to help with their floxing symptoms.
This document does not provide medical advice. It just shares information gleaned from individual experiences and is meant to provide a basic overview. Remember, just because this information is listed on this website does not mean that I, or those who contributed, promote or discourage the use of C60 Oil by floxed individuals.
Where Did this Information Come From?
Two sources. First, several years ago there was Facebook group (now defunct due to lack of activity) dedicated to C60 Oil usage. Some floxies reported tremendous remediation in their symptoms when using C60 Oil and for a while it was a fad. As with all fads it dies down. The reasons for this will be covered throughout this article. Second, from the anti-aging community who pioneered the use of C60 Oil through personal experimentation. Some people have gleaned information from the anti-aging community and modified the use for floxies. This page combines the data from the two sources.
What is C60?
C60, also known as Buckminsterfullerene or fullerene, is a molecule that consists of 60 carbon atoms, arranged as 12 pentagons and 20 hexagons. The shape is the same as that of a soccer ball. C60 molecule is a nanoparticle (very small) and is almost exactly 1 nanometer or 10 angstroms in diameter. Buckminsterfullerene is naturally found in soot and ash and fullerenes have even been detected in outer space.
C60 is frequently used in nano-technological applications, due to its high heat resistance and electrical superconductivity. C60 in its raw state or hydrated in an aqueous (water) solution is toxic. However, when you combine C60 with oil, some pretty cool stuff happens that has possible medical implications.
C60 in Oil
When C60 is mixed with olive oil (sometimes coconut MCT as well), the C60 does not dissolve; instead it forms a new compound via an ‘addition’ reaction.
Technically, it is theorized that the hydrocarbon tail of the fatty acid (olive or MCT oil) forms and adducts two fatty acids called a “bis-adduct”. It readily forms a fatty acid adduct whit is very hydrophobic but has a polar head group. The new compound C60 Oil (also called C60EVOO or C60-OO) behaves very differently than the original C60 itself. It is this C60 oil molecule that we are interested in, NOT the original C60.
This new C60 Oil, because of its lipid adduct, becomes highly likely to be incorporated into cellular membranes. This method action is poorly defined, but it acts like a phospholipid allowing it to localize into cellular membranes. This membrane localization, particularly in mitochondrial membranes, places it right where the action is, in terms of interacting with oxidative stress or ROS production. The C60 oil appears to plugs the ‘hole’ created by oxidative stress.
A famous study published in 2012 entitled “The prolongation of the lifespan of rats by repeated oral administration of fullerene” caused great interest when it documented some amazing activities of C60 oil:
Here we show that oral administration of C60 dissolved in olive oil (0.8 mg/ml) at reiterated doses (1.7 mg/kg of body weight) to rats not only does not entail chronic toxicity but it almost doubles their lifespan. The effects of C60-olive oil solutions in an experimental model of CCl4 intoxication in rat strongly suggest that the effect on lifespan is mainly due to the attenuation of age-associated increases in oxidative stress. Pharmacokinetic studies show that dissolved C60 is absorbed by the gastro-intestinal tract and eliminated in a few tens of hours.
These results of importance in the fields of medicine and toxicology should open the way for the many possible -and waited for- biomedical applications of C60 including cancer therapy, neurodegenerative disorders, and ageing.
C60 Oil a Novel Antioxidant
It appears that is C60 Oil is a super anti-oxidant but C60 Oil is very different from most molecules that we have come to think of as an “antioxidants”. For instance, it has the ability to accept and stabilize more than one electron and that is both rare and significant. Its catalytic action sets it apart from most other common antioxidants. There are those that believe the amazing effects of C60 Oil go way beyond being a super anti-oxidant. On the surface it appears that C60 Oil is a good supplement for longevity and other health benefits, but due to its short history, no one really knows for sure. There are theories that C60 Oil manipulates epigenetics.
Surfing around the internet you will find non-uniform references to C60. This often leads to confusion among those inquiring about using C60, especially from those who are uninformed. Many of those who are referring to C60 oil, C60-OO or C60EVOO actually use just C60 for short, leading some to confuse it with the original non-lipid adducted molecule. Researching C60 in its raw state can lead to some alarming information about it’s toxicity in raw form and this is why some people who stumble upon references to C60 toxicity get very alarmed.
Is C60 Oil Toxic?
As just mentioned, there is controversy about the toxicity of C60. There are many papers on the internet showing toxicity of C60. These papers usually reference C60 in its raw state or hydrated in an aqueous solution. Unmodified C60 cannot dissolve in water, only be suspended. However based on user experience, C60 oil mixtures are non-toxic as opposed to C60-water mixtures or hydroxylated C60 are toxic to varying degrees. There are no papers that we are aware of at the time of this writing that show of C60-OO being toxic.
It is important to note that due to it’s recent arrival into the science realm, the long term effects of C60 Oil in mammals are unknown, but again, it has been used extensively by certain groups since it’s inception. Once such group is a group of life span increasing enthusiasts, many of who can be found on the forum section of Longecity.org. Also, because of its apparent health benefits, a handful of companies around the world have started commercially producing C60 oil.
Here are some recent studies and papers promoting various benefits:
C60 fullerene as promising therapeutic agent for correcting and preventing skeletal muscle fatigue.
Carboxylic Acid Fullerene (C60) Derivatives Attenuated Neuroinflammatory Responses by Modulating Mitochondrial Dynamics.
Induction of different reactive oxygen species in the skin during various laser therapies and their inhibition by fullerene.
I will add more papers as I find them or others submit them to me.
Data about C60 Oil
Due to the fact that the interest in C60 oil is new (around 2012) there are very few pools of data about C60 oil. The larger pool of data about usage in humans comes from the anti-aging community1, and this data is mainly from use in healthy individuals. The smaller data came from a group of floxies who used C60 Oil in the past.
Regardless, there are NO detailed pools of data for use in a chronic health population, all the data we have has been compiled from anecdotal data over the last several years given to me by floxed folks who to C60 Oil and shared their experiences.
One of the reasons that C60 Oil lost interest in the FQ community was mainly due to a phenomenon noticed in the anti-aging community and that is a fading effect. This a phenomenon occurs in some individuals where the positive effects of the C60 Oil fade or subside. This is not an uncommon occurrence in medications and supplements, but since C60 offered such tremendous symptom remediation in some individuals, they fading effect became disheartening. However, there are some good theories as why the fading happened. In the FQ community, some individuals who were being helped by the C60 Oil continued taking the C60 Oil continuously or a larger dosages in their eagerness to have full symptom abatement. Some theorized that, from observational data, again from the anti-aging community, many of the people who reported fading were both younger and more healthy with probably less DNA damage. Also it appears that a majority of those who reported fading used C60 Oil constantly and in higher doses. However, fading was observed in some floxies who used C60 oil continuously. Did these floxies not have higher levels of DNA damage? We may never know, but we can glean some wisdom from some very smart folks who have experimented with C60 Oil extensively.
A very wise and experienced user of C60 from the anti-aging community said regarding C60 Oil,
“This is a case of longer is not better as more people have reported that the effects fade with continuous heavy use than not.”
The Fading effects seem to be mediated for some individuals by pulse or intermittent dosing, which is discussed more in the dosing section.
Fading: Digging Down Further
It is theorized that the fading effects seen in C60 Oil have to due with the depletion of stem cells.
Stem cells predominately divide asymmetrically into 2 daughter cells, one a somatic cell the other a stem cell. Theoretically this would keep the population stable, but over a lifetime it does not, and the falling population becomes a major source of aging. It happens to everyone.
The number of stem cells in young people is very much higher than older people. This allows for a better and more efficient replacement mechanism in the young contrary to the old. It is one of the reasons we see less recovery in older folks who become floxed. In other words, older folks have a lower number of good stem cells. Even in healthy people, stem cells decrease in number and tend to lose the ability to differentiate into progenies or lymphoid lineages and myeloid lineages (2).
C60 Oil stimulates mitochondria, and mitochondrial activity drives the activity of stem cells. Anecdotal reports suggest that C60 is a powerful stimulant of stem cells, but as seen in several floxed the positive effects appear to fade. This phenomenon happens in healthy people as well. It is theorized that continuous use of C60 causes the stem cell pool to become depleted.
While stem cells generally divide asymmetrically, symmetric division is also possible, wherein one stem cell divides into two stem cells, called self-renewal. It appears that mitochondria direct the process. When mitochondria are in a state of fusion, symmetric proliferation is more common, thus increasing the population of stem cells.
Changes in mitochondrial structure direct the fate of stem cells. Elongated fused mitochondria in neural stem cells maintain low ROS (oxidative stress) levels and promote self-renewal, while a transition of mitochondria to a more fragmented state (fissioned) results in a modest increase in ROS levels, thereby inducing the expression of genes that inhibit self-renewal and promote commitment and differentiation (3).
Research has shown that the metabolite stearic acid is a mitochondrial regulator. They found that animal cells are poised to respond to both increases and decreases in stearic acid levels and increased stearic acid in dietary intake can boosting mitochondrial fusion. As a side, and intriguingly fascinating, dietary stearic acid supplementation can counteract the mitochondrial dysfunction caused by genetic defects (4). It is theorized that adding stearic acid to the dosing regimen can help with the stem pool depletion by regulating the mitochondria.
Levels of C6o Oil in Serum/Tissues
According to the Baati Study the serum level falls below the limit of detection in a matter of hours, but that does not mean it has left the body. The fatty acid adduct of C60-OO is most likely incorporated into membranes like any other fatty acid. Once in the membrane, it will be there for a long time. Membrane lipids are turned over slowly, and the effective dose for C60-OO incorporated into a mitochondrial membrane is very low.
Depending on how much you have taken, it could take weeks, months, or even years for the levels of C60-OO in the membrane to drop below the effective level.
Several dosing regimens have been used, and I will list those regimens in order from the most simple to the most complex. One take away is that there is no standardized dosage. Also, keep in mind there are no double blind human studies proving either the efficacy or safety of C60 oil using any particular dosing regimen.
Before we look at the specifics of dosing we want to recap some important information. First, the half-life of C60 Oil is extremely long so some experienced users believe there is no need to dose daily. Second, there are some good theories as to why daily dosing is not recommended and pulse dosing is a safer route that helps curtail the fading effect reported by many. As we briefly looked at before, pulse dosing, to some, has to do with mitochondrial bio-genesis.
A few words of caution: It should be noted that many who have used large continuous doses were individuals who are NOT battling chronic health conditions. Starting low and slow is always recommended for sensitive users when starting any new protocol.
One of the C60 Oil manufacturers lists the following dosage information:
the most common dosage taken by people wishing to experiment with the long-term benefits of Carbon 60 is one teaspoon per day (approx 5 ml). There are about 20 teaspoons in our 100 ml bottle. After gauging their results from that baseline, many customers have reported a preference for taking two teaspoons per day (approx 10 ml), while some have dialed back their dosage to 1-3 ml per day. Carbon 60 olive oil can be taken any time of day, with or without food, depending on personal preference.
In the original, The Baati Study we mentioned earlier the following doses were administered.
- Starting at a given point:
- 1 dose every day for the first 7 days
- 1 dose per week for the next 7 weeks
- 1 dose per 2 weeks for the next 5 months
- for a total of 24 doses
Daily use: Many healthy users aim for around 0.75 to 1 mg/day when starting, and titrating to several milligrams per day.
Intermittent or Pulse Dosing (C60 Oil Alone): Starting at 1 to 2mg once per week titrating to 5-7mg once a week. Some experienced users pulse at 20-30 mg once a month.
Intermittent or Pulse Dosing (C60 Oil with stearic acid used for stem cell increase): Up to 10 g of stearic acid (120 mg/kg)* and 1 teaspoon of C60 oil half an hour later, once per week. The teaspoon of C60 Oil is about .6 mg/ml, or 3 mg C60.
Intermittent or Pulse Dosing for Advanced Users (C60 Oil with supplements for stem cells and mito biogenesis):
Taken in the morning
- Stearic acid — 10 g* (food grade, in brownie)
- Liposomal glutathione — 1 g
Taken 3 hours later
- Threonine — 5 g
- Taurine — 5 g
- Methionine — 3 g
- Lysine — 2 g
- Leucine — 5 g
- Sulforaphane — 50 mg
- PQQ — 20mg
- Liposomal glutathione — 1 g
- C60 — 3 mg (in oil)
- SAM-e — 500 mg
Notes on the advanced dosing regimen: Dosing sizes are for information only and can vary due to individual sensitivity, availability, etc…Taurine stimulates neurogenesis. Methionine is the primary nutrition for pluripotent cells, though threonine also seems necessary. So is lysine. Leucine stimulates both mito biogenesis and stem cell proliferation. Sulforaphane penetrates the BBB to produce mito fusion there, while stearic acid is blocked.
* Alternatively you can substitute 5 grams of glycerol monosterate readily available(mixing it into hot chocolate or a hot food like oatmeal). It is far more rapidly digested. If you suffer from high blood pressure this may rapidly push up blood pressure.
Where Can I Get C60?
C60 Oil can be purchased from companies or made at home. It takes some knowledge and access to certain equipment to make C60 oil properly at home. That resource will be the topic of another article. For now I will list some of the more reputable companies (Please note that I have no connection with these companies whatsoever). I will post companies who meet a certain criteria as they become known to me.
Criteria for manufacturing safe C60 oil: For C60 oil to be be of high quality it needs to be mixed for period of no less that one week (longer preferred) using specialized equipment that ensures constant suspension of Carbon 60 particles. Additionally, the mixing must occur in complete darkness (a critical factor) and done in a temperature controlled environment to avoid degradation from light or heat. The following companies meet those requirements:
There was one other brand that passed quality control and that was Good and Cheap Carbon 60 Olive Oil.
C60 is very light sensitive and the amber bottles normally used are not sufficient to protect it from oxidation just sitting out in ambient light. This is despite the fact that all the C60 Oil distributors are using them. It is recommended that the bottle be stored in darkness at all time and only brought out under minimal light for dosing. Some of the more experienced users use metal bottles and always, despite the container, store them out of light in a drawer or cabinet that stays room temperature or slightly cooler. For long term storage, covered bottles that are freezer safe then stored in the freezer. C60 oil will store for years that way. Again, at the bare minimum, if you use it in the glass bottle, keep the bottle covered and not exposed to light.
At the time of this writing I have only had contact with a relatively small group of chronically floxed folks who have seriously tried C60, compared to the totality of the community. Some have reported tremendous remediation of their toxicity symptoms, presumably due to the potent antioxidant qualities, thus there was reason for excitement. Some, who had tremendous early success ultimately reported the results fading. It is believed that some of the reports of fading occurred in those who were taking it continuously. And as always, there were some who reported no positive or negative effects (basically it did nothing for them). I have not received any reports of serious reactions to the C60 oil from any floxed folks.
Having said that, it is wise and prudent to learn as much as one can about C60 oil, understanding that this is a very new field of learning and the sources of info are limited. There are no historically experiential reports of reliability in any groups of chronically ill individuals to extrapolate the safety and efficacy of C60 oil long term. Some folks who were very sick however felt they had no other avenue to turn to, so they gave it a try. Remember, it is not officially approved for human consumption by the FDA. If you choose to experiment with C60 0il you do so at your own risk. If you are a C60 Oil user please report your findings to me pro or con, as it could benefit others.
It is important to note that even though it has been effective in helping some individuals with their floxing symptoms some theorists believe that C60 may also induce DNA mutations. Because of this some believe that C60 has a higher potential for harm with an unknown amount of risks. Reported side effects have been minimal but long term data is lacking. Again, some warn against supplementation until proper clinical trials determine the effectiveness and safety profile of this substance.
The LongeCity (Longecity.org/ImmInst.org) is an international, not-for-profit, membership-based organization (“501-3-c status” in the United States). Located at longecity.org