How to Dose Peptides
So now you have a vial of peptides, a box of syringes, and some bacteriostatic water. The next step is to reconstitute it and draw the correct amount into a syringe for injection.
Note About Online Peptide Calculators: If you use a peptide calculator make sure you still understand the math or at the very least have someone you know who can help you and double check your math. Generally, if you understand the math you’ll find you probably don’t need a peptide calculator. Note: if you don’t understand the math you can’t trust the results. Dosage calculation is a non-negotiable skill you must become comfortable with to pursue peptides safely.
Below, there are calculations in three methods of increasing complexity. Method 1 has the simplest math, but read Method 2 for an explanation of the reconstitution steps.
Essential skills: You must be able to convert milligrams to micrograms, and units to milliliters. A ‘unit’ isn’t a universal measurement, but within this document we are referring to the U-100 standard in which there are 100 units in a ml. If your 1ml syringe has marks indicating a range from 0-100, you can proceed as written. If you have smaller syringes or different markings, you’ll need to make adjustments.
100 units = 1ml
1 unit = .01ml
1mg = 1,000mcg
1mcg = .001mg
Method 1: Reconstituting with the amount of bacteriostatic water required to deliver the desired dose in 10 units.
The goal of reconstitution is to dissolve a certain weight of peptide in a volume of bacteriostatic water. Then to pull an amount of the water into a syringe that will deliver the desired weight of peptide.
The most common approach is to use a specific amount of bacteriostatic water to reconstitute, such as 2 or 3ml. Then pull the appropriate number of units into the syringe. This is convenient for adjusting doses over time, and is explained in Methods 2 & 3.
However, the simplest possible approach is to choose a dosage and the number of units you want to inject, and then reconstitute the peptide with the exact amount of water required for that concentration. This will make it easy to deliver your desired dosage, but adjusting the dose later on may require some math.
To do this you simply divide the total weight of the peptide in the vial by the desired dosage to get the number of doses in the vial. The only requirement is that they be in the same units of measurement.
Then you simply multiply the number of doses by the desired number of units. I recommend 10 units. The result is the number of units to use for reconstitution.
What you need to know:
How many milligrams (mg) in vial?
Converted to mcg:
How many micrograms (mcg) in desired dose?
Multiply mg by 1,000 to get mcg OR divide mcg by 1,000 to get mg. Either way, you must match units of measurement.
Example: 5mg vial, 250mcg dose
5mg/.250mg = 20 doses
5,000mcg/250mcg = 20 doses
20 doses X 10 units = 200 units (2ml)
So you inject 2ml (two full 1ml syringes) into the vial to reconstitute. And pull 10 units out of the vial to deliver the 250mcg dose.
Example: 2mg vial, 250mcg dose
2mg/.250mg = 8 doses
2,000mcg/250mcg = 8 doses
8 doses X 10 units = 80 units (.8ml)
So you inject .8ml (80% of a 1ml syringe) into the vial to reconstitute. And pull 10 units out of the vial to deliver the 250mcg dose.
Example: 2mg vial, 100mcg dose
2mg/.100mg = 20 doses
2,000mcg/100mcg = 20 doses
20 doses X 10 units = 200 units (2ml)
So you inject 2ml (two full 1ml syringes) into the vial to reconstitute. And pull 10 units out of the vial to deliver the 100mcg dose.
Method 2: Using 2ml to dissolve 5mg, and a chart to determine units for injection.
This example uses 2ml to dissolve 5mg. This is generally enough bacteriostatic water to dissolve the peptide, and results in 25mcg per unit. This makes the math easy, as 10 units = 250mcg.
- Use an alcohol wipe to sanitize the seals. Also sanitize the skin around the injection site.
- Draw 1ml of bacteriostatic water into the syringe.
- Inject the 1ml into the vial of peptides. It may be best to aim for the wall of the vial and be gentle, it may not matter at all. Before withdrawing the needle, pull 1ml of air into the syringe. This will equalize the pressure.
- Inject the 1ml air into the bacteriostatic water vial, equalizing pressure.
- Repeat steps 2-4. You should now have 2ml of bacteriostatic water in the peptide vial.
- Gently roll the vial, or wait for the peptides to dissolve.
- Use the chart below and draw your desired dosage into the syringe.
- Here is a video of subcutaneous injection into belly fat.
|Number of units
||Dosage (when 5mg is dissolved in 2ml)
Method 3. Using 3ml to dissolve 5mg, and a chart to determine units for injection.
I often use 3ml to dissolve 5mg. It’s always enough to dissolve the peptide, and I prefer working with a little more liquid in each dose. I’ve included more math to give you the tools to navigate other situations.
I’m going to explain what to do if your peptide vial is 5mg (a measurement of weight equivalent to 5,000mcg), your syringes are 1ml (volume equivalent to 1cc), and your desired dosage is 250mcg (equal to .25mg). Steps 6, 7, & 8 are optional, but intended to give you the tools to navigate other scenarios. Math results are rounded for clarity.
- Wipe all the seals with an alcohol pad, and the area of skin where you’ll be injecting.
- Draw 1ml of bacteriostatic water into the syringe by pulling it back to the 100 units marking.
- Gently spray the 1ml into the vial of peptides, preferably aimed at the wall of the vial.
- Before removing the syringe, draw 1ml of air into the syringe. This will restore neutral pressure.
- Inject the 1ml of air into the bacteriostatic water container (neutralizing pressure), and repeat steps 2-5 until there are 3ml in the peptide vial.
- Get out a calculator. We now have 5mg dissolved in 3ml. So divide 5 by 3 to get 1.6667. That’s how many mg are in each 1ml. We want to know what percentage of a ml is necessary to deliver 250mcg (.250mg). So divide .25 by 1.6667 and you’ll get .15, or 15%. Our 1ml syringe has 100 units, so 15% is 15 units, or .15ml
- To double check your math, again divide 5mg by 3ml to get 1.6667, but this time multiply it by .15. The result should be .25, representing the .25mg (250mcg) of peptide in 15 units.
- Repeat steps 6 & 7 until it clicks. Test yourself with these scenarios if you want to be sure you understand. Answers at the end of the doc (rounded for clarity).
- If you dissolve 5mg in 3ml, and want a dosage of 200mcg.. How many units will you draw into the syringe for injection?
- If you dissolve 5mg in 3ml, and draw 20 units into the syringe.. How many mcg will that deliver?
- If you dissolve 2mg in 2ml, and want a dosage of 250mcg.. How many units will you draw into the syringe for injection?
- Oops, you ordered .5ml syringes, the markings go up to 50 units. How many times will you fill the syringe to transfer 2ml?
- The syringes you ordered are 1ml/1cc, but the markings go from .1 up to 1.0.. You originally wanted 25 units, what will the equivalent be on this syringe?
- Once the peptide is fully dissolved, and you’re confident in your math, draw 15 units into the syringe. This will contain 250mcg of the peptide if the vial had 5mg, and was reconstituted with 3ml.
- Use an alcohol wipe on the injection site if you haven’t already. Here is a video of subcutaneous injection into belly fat.
|Number of units
||Dosage (when 5mg is dissolved in 3ml)
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